Extraction, purification and characterization of bioactive phytochemicals (phenolics, terpenoids, alkaloids, carbohydrates and organic acids) and biochemical fingerprinting of metabolites using RP-SPE, HPLC, UPLC, LC-MS/MS, ESI-QTOF-MS and NMR techniques. Metabolomics study of bioactives using LC techniques. Development and validation of analytical HPLC methods using different stationary phases for evaluation of bioactive phytochemicals.
Development of green and sustainable process technologies for bioresource valorization and formulation of diversified value added products; functional beverages (alcoholic and non-alcoholic) and nutraceuticals from underutilized agro-biomass. Quality and stability evaluation of nutraceuticals, herbal extracts and functional beverages. Process optimization from lab scale to pilot facility
DEVELOPED PRODUCT/PROCESS TECHNOLOGIES
1. Developed a green and sustainable technology for purification of green tea polyphenols (Catechins)*
Technology transferred:
1. M/s Baijnath Pharmaceutical Pvt. Ltd. Paprola, Himachal Pradesh
2. The INDCOSERVE- Coonor Tea Trade Association, Nilgiris, Tamil Nadu
2. Developed a sustainable process technology for production of black tea polyphenols (Theaflavins)
3. Developed a new sustainable process technology for manufacturing of high quality green tea with enhanced flavour
4. Developed a green process based technology for production of bio-amino acids from tea and other bioresources
5. Developed a new process for preparation of tea concentrates and instant teas
6. Developed process technology for preparation of tea and tea based wines
MOU Signed with SDZ Cha Sarl Mozambique
RESEARCH ARTICLES/PUBLICATIONS
1. Rana, A., Dhyani, D., Gulati A., Singh, H. P. 2011. Isolation of two major iridoid glucosides from Incarvillea emodi. Natural Product Research, 25 (10): 1014-1017.
2. Rana, A., Singh, H. P., Dhyani, D. 2012. Comparative estimation of major iridoid glucosides from different parts of Incarvillea emodi. ISRN Chromatography, DOI:10.5402/2012/783752.
3. Rana, A., Singh, H. P. 2012. A rapid HPLC-DAD method for analysis of theaflavins, using C12 as stationary phase. Journal of Liquid Chromatography and Related Technologies, 35: 2272–2279.
4. Rana, A., Singh, H. P. 2013. Bio-utilization of wild Berries for manufacturing of highly valued herbal wines. Indian Journal of Natural Products and Resources, 4 (2): 165-169.
5. Rana, A., Bhangalia, S., Singh, H. P. 2013. A new phenylethanoid glucoside from Jacaranda mimosifolia. Natural Product Research, 27 (13): 1167-1173.
6. Rana, A., Rana, S., Majeed, R., Singh, H. P., Gulati, A., Hamid, A., Vyas, D., Dhyani, D. 2014. Comparative studies for screening of bioactive constituents from various parts of Incarvillea emodi. Natural Product Research, 28 (8): 593-596.
7. Rana, S., Rana, A., Gulati, A., Bhushan, S. 2014. RP-HPLC-DAD determination of phenolics in industrial apple pomace. Food Analytical Methods, 7:1424–1432.
8. Rana, A., Singh, H. P., Gulati, A. 2015. Concurrent analysis of theanine, caffeine and catechins using hydrophobic selective C12 stationary phase. Journal of Liquid Chromatography and Related Technologies.38: 709-715.
9. Joshi, R., Rana, A., Gulati A. 2015. Studies on quality of orthodox teas made from anthocyanins rich tea clones growing in Kangra Valley, India. Food Chemistry. 176: 357-366.
10. Rana, A., Singh, H. P., Gulati, A. 2015.Investigation of major phenolic antioxidants from Camellia sinensis Fruits. Cogent Chemistry, Vol. 1, Issue 1.
11. Rana, A., Kumar, D., Joshi, R., Gulati, A., Singh, H. P. 2015. In vitro cytotoxicity of black tea theaflavin digallates against Chinese hamster ovary cell lines (CHOK1) and glioma cell lines (C-6). Chemistry of Natural Compounds. 51: 835-839.
12. Rana, S., Gupta, S., Rana, A., Bhushan, S. 2015. Functional properties, phenolic constituents and antioxidant potential of industrial apple pomace for utilization as active food ingredient. Food Science and Human Wellness. 4: 180-187.
13. Rana, S., Kumar, S., Rana, A., Sharma, V., Katoch, P., Padwad, Y., Bhushan, S. 2016. Phenolic Constituents from Apple Tree Leaves and their in vitro Biological Activity. Industrial Crops and Products. 90: 118-125.
14. Rana, A*., Sharma, E., Rawat, K., Sharma, R., Verma, S., Padwad, Y.S., Gulati, A. 2016. Screening and Purification of Catechins from Underutilized Tea Plant Parts and Their Bioactivity Studies. Journal of Food Science and Technology. 53(11):4023–4032.
15. Joshi, R., Rana, A., Kumar, V., Kumar, D., Padwad, Y.S., Yadav, S.K., Gulati, A. 2017. Anthocyanins Enriched Purple Tea Exhibits Antioxidant, Immuno-Stimulatory and Anticancer Activities. Journal of Food Science and Technology. 54 (7): 1953-1963.
16. Shrama, R., Verma, S., Rana, S., Rana, A.* 2018. Rapid Screening and Quantification of Major Organic Acids in Citrus Fruits and their Bioactivity Stuides. Journal of Food Science and Technology. Accepted
